Rocco D'Antuono

Rocco D'Antuono

Microscopist & image analyst

Confocal, multiphoton and intravital

Wide-field, TIRF

FLIM, FCS

Imaging platforms: Leica, Zeiss, Olympus, Nikon, LaVision BioTech, BD

Programming: ImageJ macro, μManager beanshell, Java, python, R, html


Bio

Biophysics education at Laboratory for Advanced BioSpectroscopy (UniMiB)

Microscope trainer, image acquisition and image analysis teaching:

  • PhD course, European School of Molecular Medicine, 2012-2014, IFOM-IEO Campus (Milan, IT)
  • Leica Confocal school, 2012-2014, Fondazione Filarete (Milan, IT)
  • PhD course, Microscopy application for immunological research, 2014-2017, ProDoc, Uni of Bern/IRB-USI (CH)
  • FACS & sorting operator (2014-2017), Mass Spec beginner (2017).

    EUBIAS (2013) and NEUBIAS son (2017-2019) and trainer (TS12, 2019).

    NEUBIAS Academy organization committee (2020)


    Current role:

    Senior Microscopist

    Crick Advanced Light Microscopy, The Francis Crick Institute, London (UK)

    CALM website banner
    from CALM website

    Previous role: 2014-2017

    Imaging facility

    Institute for Research in Biomedicine, Bellinzona (CH)

    gut_crypts_Perruzza_NatComm-2019
    Gut crypts 3D reconstruction
    Nat. Comms. 2019

    Previous role: 2011-2014

    Microscopy facility

    IFOM, FIRC Institute for Molecular Medicine, Milan (IT)

    Focal_adhesions_TIRF_Marchesi_DevCell_2014.png
    Focal adhesions - TIRF
    Dev. Cell. 2014

    Education:

    MSc Physics, Prof. Giuseppe Chirico, Laboratory for Advanced BioSpectroscopy,

    University of Milano-Bicocca, Milan (IT)


    BSc Physics, University of Milano-Bicocca, Milan (IT)

    thermocamera_RDA2018
    University is also fun


    Publications:

    CDCP1 overexpression drives prostate cancer progression and can be targeted in vivo - J Clin Invest. 2020;130(5):2435-2450
    Abdullah Alajati, Mariantonietta D'Ambrosio, Martina Troiani, Simone Mosole, Laura Pellegrini, Jingjing Chen, Ajinkya Revandkar, Marco Bolis, Jean-Philippe Theurillat, Ilaria Guccini, Marco Losa, Arianna Calcinotto, Gaston De Bernardis, Emiliano Pasquini, Rocco D'Antuono, Adam Sharp, Ines Figueiredo, Daniel Nava Rodrigues, Jonathan Welti, Veronica Gil, Wei Yuan, Tatjana Vlajnic, Lukas Bubendorf, Giovanna Chiorino, Letizia Gnetti, Verónica Torrano, Arkaitz Carracedo, Laura Camplese, Susumu Hirabayashi, Elena Canato, Gianfranco Pasut, Monica Montopoli, Jan Hendrik Rüschoff, Peter Wild, Holger Moch, Johann De Bono, and Andrea Alimonti

    Protection against influenza infection requires early recognition by inflammatory dendritic cells through C-type lectin receptor SIGN-R1 - Nature Microbiology volume 4, pages1930-1940(2019)
    Miguel Palomino-Segura, Laurent Perez, Yagmur Farsakoglu, Tommaso Virgilio, Irene Latino, Rocco D'Antuono, Nikolaos Chatziandreou, Diego U. Pizzagalli, Guojun Wang, Adolfo García-Sastre, Federica Sallusto, Michael C. Carroll, Olivier Neyrolles & Santiago F. Gonzalez

    ATP released by intestinal bacteria limits the generation of protective IgA against enteropathogens - Nat. Comm. 10, (2019)
    Michele Proietti, Lisa Perruzza, Daniela Scribano, Giovanni Pellegrini, Rocco D'Antuono, Francesco Strati, Marco Raffaelli, Santiago F. Gonzalez, Marcus Thelen, Wolf-Dietrich Hardt, Emma Slack, Mauro Nicoletti & Fabio Grassi

    Re-education of Tumor-Associated Macrophages by CXCR2 Blockade Drives Senescence and Tumor Inhibition in Advanced Prostate Cancer - Cell Reports, Vol.22, Issue 8, Pages 2156-2168.e5 (2019)
    Diletta Di Mitri, Michela Mirenda, Jelena Vasilevska, Arianna Calcinotto, Nicolas Delaleu, Ajinkya Revandkar,Veronica Gil, Gunther Boysen, Marco Losa, Simone Mosole, Emiliano Pasquini, Rocco D'Antuono, Michela Masetti, Elena Zagato, Giovanna Chiorino, Paola Ostano, Andrea Rinaldi, Letizia Gnetti, Mariona Graupera, Ana Raquel Martins Figueiredo Fonseca, Ricardo Pereira Mestre, David Waugh, Simon Barry, Johann De Bono, Andrea Alimonti

    Epigenetic Control of Mitochondrial Fission Enables Self-Renewal of Stem-like Tumor Cells in Human Prostate Cancer - Cell Metabolism, Vol. 30, Issue 2, Pages 303-318.e6 (2019)
    Gianluca Civenni, Roberto Bosotti, Andrea Timpanaro, Ramiro Vàzquez, Jessica Merulla, Shusil Pandit, Simona Rossi, Domenico Albino, Sara Allegrini, Abhishek Mitra, Sarah N. Mapelli, Luca Vierling, Martina Giurdanella, Martina Marchetti, Alyssa Paganoni, Andrea Rinaldi, Marco Losa, Enrica Mira-Catò, Rocco D'Antuono, Diego Morone, Keyvan Rezai, Gioacchino D'Ambrosio, L'Houcine Ouafi, Sarah Mackenzie, Maria E.Riveiro, Esteban Cvitkovic, Giuseppina M.Carbone, Carlo V.Catapano

    ER-to-lysosome-associated degradation of proteasome-resistant ATZ polymers occurs via receptor-mediated vesicular transport - The EMBO Journal, e99259 (2018)
    Ilaria Fregno, Elisa Fasana, Timothy J Bergmann, Andrea Raimondi, Marisa Loi, Tatiana Soldà, Carmela Galli, Rocco D'Antuono, Diego Morone, Alberto Danieli, Paolo Paganetti, Eelco van Anken, Maurizio Molinari

    Compartmentalized activities of the pyruvate dehydrogenase complex sustain lipogenesis in prostate cancer - Nature Genetics, Vol. 50, pages219-228 (2018)
    Jingjing Chen, Ilaria Guccini, Diletta Di Mitri, Daniela Brina, Ajinkya Revandkar, Manuela Sarti, Emiliano Pasquini, Abdullah Alajati, Sandra Pinton, Marco Losa, Gianluca Civenni, Carlo V. Catapano, Jacopo Sgrignani, Andrea Cavalli, Rocco D'Antuono, John M. Asara, Andrea Morandi, Paola Chiarugi, Sara Crotti, Marco Agostini, Monica Montopoli, Ionica Masgras, Andrea Rasola, Ramon Garcia-Escudero, Nicolas Delaleu, Andrea Rinaldi, Francesco Bertoni, Johann de Bono, Arkaitz Carracedo & Andrea Alimonti

    Mitochondrial dysfunction induced by a SH2 domain-targeting STAT3 inhibitor leads to metabolic synthetic lethality in cancer cells - Proc Natl Acad Sci U S A (2017)
    D. Genini, L. Brambilla, E. Laurini, J. Merulla, G. Civenni, S. Pandit, R. D'Antuono, L. Perez, D. E. Levy, S. Pricl, G. M. Carbone, C. V. Catapano

    Macrophage Death following Influenza Vaccination Initiates the Inflammatory Response that Promotes Dendritic Cell Function in the Draining Lymph Node - Cell Rep (2017) vol. 18 pp2427-2440
    N. Chatziandreou, Y. Farsakoglu, M. Palomino-Segura, R. D'Antuono, D. U. Pizzagalli, F. Sallusto, V. Lukacs-Kornek, M. Uguccioni, D. Corti, S. J. Turley, A. Lanzavecchia, M. C. Carroll, S. F. Gonzalez

    Inhibition of Notch pathway arrests PTEN-deficient advanced prostate cancer by triggering p27-driven cellular senescence - Nat Commun (2016) vol. 7 pp13719
    A. Revandkar, M. L. Perciato, A. Toso, A. Alajati, J. Chen, H. Gerber, M. Dimitrov, A. Rinaldi, N. Delaleu, E. Pasquini, R. D'Antuono, S. Pinton, M. Losa, L. Gnetti, A. Arribas, P. Fraering, F. Bertoni, A. Nepveu, A. Alimonti

    Translocon component Sec62 acts in endoplasmic reticulum turnover during stress recovery - Nat Cell Biol (2016) vol. 18 pp1173-1184
    F. Fumagalli, J. Noack, T. J. Bergmann, E. C. Presmanes, G. B. Pisoni, E. Fasana, I. Fregno, C. Galli, M. Loi, T. Solda, R. D'Antuono, A. Raimondi, M. Jung, A. Melnyk, S. Schorr, A. Schreiber, L. Simonelli, L. Varani, C. Wilson-Zbinden, O. Zerbe, K. Hofmann, M. Peter, M. Quadroni, R. Zimmermann, M. Molinari

    Reduced DNA methylation and hydroxymethylation in patients with systemic mastocytosis - Eur J Haematol (2015) vol. 95 pp566-75
    C. Leoni, S. Montagner, L. Deho, R. D'Antuono, G. De Matteis, A. V. Marzano, S. Merante, E. M. Orlandi, R. Zanotti, S. Monticelli

    DEPDC1B Coordinates De-adhesion Events and Cell-Cycle Progression at Mitosis - Dev Cell (2014) vol. 31 pp420-433
    S. Marchesi, F. Montani, G. Deflorian, R. D'Antuono, A. Cuomo, S. Bologna, C. Mazzoccoli, T. Bonaldi, P. P. Di Fiore, F. Nicassio

    Tumour-infiltrating Gr-1+ myeloid cells antagonize senescence in cancer - Nature (2014) vol. 515 pp134-7
    D. Di Mitri, A. Toso, J. J. Chen, M. Sarti, S. Pinton, T. R. Jost, R. D'Antuono, E. Montani, R. Garcia- Escudero, I. Guccini, S. Da Silva-Alvarez, M. Collado, M. Eisenberger, Z. Zhang, C. Catapano, F. Grassi, A. Alimonti

    Targeting endothelial junctional adhesion molecule-A/ EPAC/ Rap-1 axis as a novel strategy to increase stem cell engraftment in dystrophic muscles - EMBO Mol Med (2014) vol. 6 pp239-58
    M. Giannotta, S. Benedetti, F. S. Tedesco, M. Corada, M. Trani, R. D'Antuono, Q. Millet, F. Orsenigo, B. G. Galvez, G. Cossu, E. Dejana

    A molecular thermometer for nanoparticles for optical hyperthermia - Nano Lett (2013) vol. 13 pp2004-10
    S. Freddi, L. Sironi, R. D'Antuono, D. Morone, A. Dona, E. Cabrini, L. D'Alfonso, M. Collini, P. Pallavicini, G. Baldi, D. Maggioni, G. Chirico



    Aknowlegments:

    Modular microfluidics enables kinetic insight from time-resolved cryo-EM
    Mäeots M., Lee B., Nans A. et al., Modular microfluidics enables kinetic insight from time-resolved cryo-EM. Nat Commun 11, 3465 (2020)

    Restriction of memory B cell differentiation at the germinal center B cell positive selection stage
    Toboso-Navasa A., Gunawan A., Morlino G. et al., J Exp Med 6 July 2020; 217 (7): e20191933

    A trainable clustering algorithm based on shortest paths from density peaks
    Pizzagalli et al., Science Advances 30 Oct 2019: Vol. 5, no. 10, eaax3770

    Influenza Vaccination Induces NK-Cell-Mediated Type-II IFN Response that Regulates Humoral Immunity in an IL-6-Dependent Manner
    Farsakoglu et al., Cell Reports, Vol.26, Issue 9 (2019)

    P2X7 receptor restrains pathogenic Tfh cell generation in systemic lupus erythematosus
    Faliti C.E. et al., J. Exp. Med. 2019 Vol. 216 No. 2 317–336

    A shortest-path based clustering algorithm for joint human-machine analysis of complex datasets
    Pizzagalli et al., https://arxiv.org/abs/1812.11850

    IL-23 secreted by myeloid cells drives castration-resistant prostate cancer
    Calcinotto et al., Nature, Vol.559 (2018)

    Leukocyte Tracking Database, a collection of immune cell tracks from intravital 2-photon microscopy videos
    Pizzagalli D.U., Scientific Data volume 5, Article number: 180129 (2018)

    A Human Bi-specific Antibody against Zika Virus with High Therapeutic Potential
    Wang et al., Cell, Vol. 171, Issue 1, 21 September 2017, Pages 229-241.e15

    Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization
    Cecchinato et al., J Immunol January 1, 2017, 198 (1) 184-195