Rocco D'Antuono

Rocco D'Antuono

Microscopist & image analyst

Confocal, multiphoton and intravital

Wide-field, TIRF

FLIM, FCS

Imaging platforms: Leica, Zeiss, Olympus, Nikon, LaVision BioTech, BD

Programming: ImageJ macro, μManager beanshell, Java, python, R, html


Contacts:
rocco.dantuono@crick.ac.uk
https://www.linkedin.com/in/rocco-dantuono/

Bio

Biophysics education at Laboratory for Advanced BioSpectroscopy (UniMiB)

Microscope trainer, image acquisition and image analysis teaching:

  • PhD course, European School of Molecular Medicine, 2012-2014, IFOM-IEO Campus (Milan, IT)
  • Leica Confocal school, 2012-2014, Fondazione Filarete (Milan, IT)
  • PhD course, Microscopy application for immunological research, 2014-2017, ProDoc, Uni of Bern/IRB-USI (CH)
  • Introduction to Confocal and Multiphoton Microscopy, 2018-2024, The Francis Crick Institute, London (UK)
  • Course on Optical Microscopy Imaging for Biosciences, 2023, i3S, Porto (PT)
  • Image data science with Python and Napari, 2023, Latin America Bioimaging (LABI) (CL)
  • Introduction to napari, 2023-2024, The Francis Crick Institute, London (UK)
  • FACS & sorting operator (2014-2017), Mass Spec beginner (2017).

    EUBIAS (2013) and NEUBIAS son (2017-2019) and trainer (TS12, 2019).

    NEUBIAS Academy organization committee (2020-2021)

    Data Analysis in IMaging (DAIM) RMS Section committee (2021-2024)

    Global BioImage Analysts' Society (GloBIAS) Founder and Steering Committee member (2023-2024)

    Scientific society memberships: Royal Microscopical Society, European Microscopy Society, Institute of Physics, IEEE


    Current role:

    Principal Microscopist and Image Analyst

    Crick Advanced Light Microscopy, The Francis Crick Institute, London (UK)

    CALM website banner
    from CALM website

    Previous role: 2018-2023

    Senior Microscopist

    Crick Advanced Light Microscopy, The Francis Crick Institute, London (UK)

    CALM website banner
    from CALM website

    Previous role: 2014-2017

    Imaging facility

    Institute for Research in Biomedicine, Bellinzona (CH)

    gut_crypts_Perruzza_NatComm-2019
    Gut crypts 3D reconstruction
    Nat. Comms. 2019

    Previous role: 2011-2014

    Microscopy facility

    IFOM, FIRC Institute for Molecular Medicine, Milan (IT)

    Focal_adhesions_TIRF_Marchesi_DevCell_2014.png
    Focal adhesions - TIRF
    Dev. Cell. 2014

    Education:

    MSc Physics, Prof. Giuseppe Chirico, Laboratory for Advanced BioSpectroscopy,

    University of Milano-Bicocca, Milan (IT)


    BSc Physics, University of Milano-Bicocca, Milan (IT)

    thermocamera_RDA2018
    University is also fun


    Publications:

    Endothelial AHR activity prevents lung barrier disruption in viral infection - Nature 621, 813–820 (2023)
    Jack Major, Stefania Crotta, Katja Finsterbusch, Probir Chakravarty, Kathleen Shah, Bruno Frederico, Rocco D’Antuono, Mary Green, Lucy Meader, Alejandro Suarez-Bonnet, Simon Priestnall, Brigitta Stockinger & Andreas Wack

    Photoablation at single cell resolution and its application in the Drosophila epidermis and peripheral nervous system - Front. Physiol., 04 January 2023
    Federica Mangione, Rocco D’Antuono, Nicolas Tapon

    Basic Digital Image Acquisition, Design, Processing, Analysis, Management, and Presentation - In: Nechyporuk-Zloy, V. (eds) Principles of Light Microscopy: From Basic to Advanced . Springer, Cham., 2022
    Rocco D'Antuono

    Fluorescence fluctuation-based super-resolution microscopy: Basic concepts for an easy start - J. Microsc.2022;288:218–241
    Alma Alva, Eduardo Brito-Alarcón, Alejandro Linares, Esley Torres-García, Haydee O. Hernández, Raúl Pinto-Cámara, Damián Martínez, Paul Hernández-Herrera, Rocco D'Antuono, Christopher Wood, Adán Guerrero

    Towards super-resolved terahertz microscopy for cellular imaging - J. Microsc.2022;288:207–217
    Rocco D'Antuono, John W. Bowen

    Extending resolution within a single imaging frame - Nat Commun 13, 7452 (2022)
    Esley Torres-García, Raúl Pinto-Cámara, Alejandro Linares, Damián Martínez, Víctor Abonza, Eduardo Brito-Alarcón, Carlos Calcines-Cruz, Gustavo Valdés-Galindo, David Torres, Martina Jabloñski, Héctor H. Torres-Martínez, José L. Martínez, Haydee O. Hernández, José P. Ocelotl-Oviedo, Yasel Garcés, Marco Barchi, Rocco D’Antuono, Ana Bošković, Joseph G. Dubrovsky, Alberto Darszon, Mariano G. Buffone, Roberto Rodríguez Morales, Juan Manuel Rendon-Mancha, Christopher D. Wood, Armando Hernández-García, Diego Krapf, Álvaro H. Crevenna & Adán Guerrero

    GIANI – open-source software for automated analysis of 3D microscopy images - J Cell Sci (2022) 135 (10): jcs259511
    David J. Barry, Claudia Gerri, Donald M. Bell, Rocco D'Antuono, Kathy K. Niakan

    ZELDA: A 3D Image Segmentation and Parent-Child Relation Plugin for Microscopy Image Analysis in napari - Front. Comput. Sci., 2022
    Rocco D’Antuono and Giuseppina Pisignano

    Multilayered regulation of autophagy by the Atg1 kinase orchestrates spatial and temporal control of autophagosome formation - Molecular Cell 81, 5066–5081
    Anne Schreiber, Ben C. Collins, Colin Davis, Radoslav I. Enchev, Angie Sedra, Rocco D’Antuono, Ruedi Aebersold, Matthias Peter

    Highlights from the 2016-2020 NEUBIAS training schools for Bioimage Analysts: a success story and key asset for analysts and life scientists [version 1; peer review: 2 approved] - F1000Research 2021, 10:334
    Gabriel G. Martins, Fabrice P. Cordelières, Julien Colombelli, Rocco D’Antuono, Ofra Golani, Romain Guiet, Robert Haase, Anna H. Klemm, Marion Louveaux, Perrine Paul-Gilloteaux, Jean-Yves Tinevez, Kota Miura

    Neutrophil subtypes shape HIV-specific CD8 T-cell responses after vaccinia virus infection - NPJ Vaccines, 2021 Apr 12;6(1):52
    Mauro Di Pilato, Miguel Palomino-Segura, Ernesto Mejías-Pérez, Carmen E Gómez, Andrea Rubio-Ponce, Rocco D'Antuono, Diego Ulisse Pizzagalli, Patricia Pérez, Raphael Kfuri-Rubens, Alberto Benguría, Ana Dopazo, Iván Ballesteros, Carlos Oscar S Sorzano, Andrés Hidalgo, Mariano Esteban, Santiago F Gonzalez

    CDCP1 overexpression drives prostate cancer progression and can be targeted in vivo - J Clin Invest. 2020;130(5):2435-2450
    Abdullah Alajati, Mariantonietta D'Ambrosio, Martina Troiani, Simone Mosole, Laura Pellegrini, Jingjing Chen, Ajinkya Revandkar, Marco Bolis, Jean-Philippe Theurillat, Ilaria Guccini, Marco Losa, Arianna Calcinotto, Gaston De Bernardis, Emiliano Pasquini, Rocco D'Antuono, Adam Sharp, Ines Figueiredo, Daniel Nava Rodrigues, Jonathan Welti, Veronica Gil, Wei Yuan, Tatjana Vlajnic, Lukas Bubendorf, Giovanna Chiorino, Letizia Gnetti, Verónica Torrano, Arkaitz Carracedo, Laura Camplese, Susumu Hirabayashi, Elena Canato, Gianfranco Pasut, Monica Montopoli, Jan Hendrik Rüschoff, Peter Wild, Holger Moch, Johann De Bono, and Andrea Alimonti

    Protection against influenza infection requires early recognition by inflammatory dendritic cells through C-type lectin receptor SIGN-R1 - Nature Microbiology volume 4, pages1930-1940(2019)
    Miguel Palomino-Segura, Laurent Perez, Yagmur Farsakoglu, Tommaso Virgilio, Irene Latino, Rocco D'Antuono, Nikolaos Chatziandreou, Diego U. Pizzagalli, Guojun Wang, Adolfo García-Sastre, Federica Sallusto, Michael C. Carroll, Olivier Neyrolles & Santiago F. Gonzalez

    ATP released by intestinal bacteria limits the generation of protective IgA against enteropathogens - Nat. Comm. 10, (2019)
    Michele Proietti, Lisa Perruzza, Daniela Scribano, Giovanni Pellegrini, Rocco D'Antuono, Francesco Strati, Marco Raffaelli, Santiago F. Gonzalez, Marcus Thelen, Wolf-Dietrich Hardt, Emma Slack, Mauro Nicoletti & Fabio Grassi

    Re-education of Tumor-Associated Macrophages by CXCR2 Blockade Drives Senescence and Tumor Inhibition in Advanced Prostate Cancer - Cell Reports, Vol.22, Issue 8, Pages 2156-2168.e5 (2019)
    Diletta Di Mitri, Michela Mirenda, Jelena Vasilevska, Arianna Calcinotto, Nicolas Delaleu, Ajinkya Revandkar,Veronica Gil, Gunther Boysen, Marco Losa, Simone Mosole, Emiliano Pasquini, Rocco D'Antuono, Michela Masetti, Elena Zagato, Giovanna Chiorino, Paola Ostano, Andrea Rinaldi, Letizia Gnetti, Mariona Graupera, Ana Raquel Martins Figueiredo Fonseca, Ricardo Pereira Mestre, David Waugh, Simon Barry, Johann De Bono, Andrea Alimonti

    Epigenetic Control of Mitochondrial Fission Enables Self-Renewal of Stem-like Tumor Cells in Human Prostate Cancer - Cell Metabolism, Vol. 30, Issue 2, Pages 303-318.e6 (2019)
    Gianluca Civenni, Roberto Bosotti, Andrea Timpanaro, Ramiro Vàzquez, Jessica Merulla, Shusil Pandit, Simona Rossi, Domenico Albino, Sara Allegrini, Abhishek Mitra, Sarah N. Mapelli, Luca Vierling, Martina Giurdanella, Martina Marchetti, Alyssa Paganoni, Andrea Rinaldi, Marco Losa, Enrica Mira-Catò, Rocco D'Antuono, Diego Morone, Keyvan Rezai, Gioacchino D'Ambrosio, L'Houcine Ouafi, Sarah Mackenzie, Maria E.Riveiro, Esteban Cvitkovic, Giuseppina M.Carbone, Carlo V.Catapano

    ER-to-lysosome-associated degradation of proteasome-resistant ATZ polymers occurs via receptor-mediated vesicular transport - The EMBO Journal, e99259 (2018)
    Ilaria Fregno, Elisa Fasana, Timothy J Bergmann, Andrea Raimondi, Marisa Loi, Tatiana Soldà, Carmela Galli, Rocco D'Antuono, Diego Morone, Alberto Danieli, Paolo Paganetti, Eelco van Anken, Maurizio Molinari

    Compartmentalized activities of the pyruvate dehydrogenase complex sustain lipogenesis in prostate cancer - Nature Genetics, Vol. 50, pages219-228 (2018)
    Jingjing Chen, Ilaria Guccini, Diletta Di Mitri, Daniela Brina, Ajinkya Revandkar, Manuela Sarti, Emiliano Pasquini, Abdullah Alajati, Sandra Pinton, Marco Losa, Gianluca Civenni, Carlo V. Catapano, Jacopo Sgrignani, Andrea Cavalli, Rocco D'Antuono, John M. Asara, Andrea Morandi, Paola Chiarugi, Sara Crotti, Marco Agostini, Monica Montopoli, Ionica Masgras, Andrea Rasola, Ramon Garcia-Escudero, Nicolas Delaleu, Andrea Rinaldi, Francesco Bertoni, Johann de Bono, Arkaitz Carracedo & Andrea Alimonti

    Mitochondrial dysfunction induced by a SH2 domain-targeting STAT3 inhibitor leads to metabolic synthetic lethality in cancer cells - Proc Natl Acad Sci U S A (2017)
    D. Genini, L. Brambilla, E. Laurini, J. Merulla, G. Civenni, S. Pandit, R. D'Antuono, L. Perez, D. E. Levy, S. Pricl, G. M. Carbone, C. V. Catapano

    Macrophage Death following Influenza Vaccination Initiates the Inflammatory Response that Promotes Dendritic Cell Function in the Draining Lymph Node - Cell Rep (2017) vol. 18 pp2427-2440
    N. Chatziandreou, Y. Farsakoglu, M. Palomino-Segura, R. D'Antuono, D. U. Pizzagalli, F. Sallusto, V. Lukacs-Kornek, M. Uguccioni, D. Corti, S. J. Turley, A. Lanzavecchia, M. C. Carroll, S. F. Gonzalez

    Inhibition of Notch pathway arrests PTEN-deficient advanced prostate cancer by triggering p27-driven cellular senescence - Nat Commun (2016) vol. 7 pp13719
    A. Revandkar, M. L. Perciato, A. Toso, A. Alajati, J. Chen, H. Gerber, M. Dimitrov, A. Rinaldi, N. Delaleu, E. Pasquini, R. D'Antuono, S. Pinton, M. Losa, L. Gnetti, A. Arribas, P. Fraering, F. Bertoni, A. Nepveu, A. Alimonti

    Translocon component Sec62 acts in endoplasmic reticulum turnover during stress recovery - Nat Cell Biol (2016) vol. 18 pp1173-1184
    F. Fumagalli, J. Noack, T. J. Bergmann, E. C. Presmanes, G. B. Pisoni, E. Fasana, I. Fregno, C. Galli, M. Loi, T. Solda, R. D'Antuono, A. Raimondi, M. Jung, A. Melnyk, S. Schorr, A. Schreiber, L. Simonelli, L. Varani, C. Wilson-Zbinden, O. Zerbe, K. Hofmann, M. Peter, M. Quadroni, R. Zimmermann, M. Molinari

    Reduced DNA methylation and hydroxymethylation in patients with systemic mastocytosis - Eur J Haematol (2015) vol. 95 pp566-75
    C. Leoni, S. Montagner, L. Deho, R. D'Antuono, G. De Matteis, A. V. Marzano, S. Merante, E. M. Orlandi, R. Zanotti, S. Monticelli

    DEPDC1B Coordinates De-adhesion Events and Cell-Cycle Progression at Mitosis - Dev Cell (2014) vol. 31 pp420-433
    S. Marchesi, F. Montani, G. Deflorian, R. D'Antuono, A. Cuomo, S. Bologna, C. Mazzoccoli, T. Bonaldi, P. P. Di Fiore, F. Nicassio

    Tumour-infiltrating Gr-1+ myeloid cells antagonize senescence in cancer - Nature (2014) vol. 515 pp134-7
    D. Di Mitri, A. Toso, J. J. Chen, M. Sarti, S. Pinton, T. R. Jost, R. D'Antuono, E. Montani, R. Garcia- Escudero, I. Guccini, S. Da Silva-Alvarez, M. Collado, M. Eisenberger, Z. Zhang, C. Catapano, F. Grassi, A. Alimonti

    Targeting endothelial junctional adhesion molecule-A/ EPAC/ Rap-1 axis as a novel strategy to increase stem cell engraftment in dystrophic muscles - EMBO Mol Med (2014) vol. 6 pp239-58
    M. Giannotta, S. Benedetti, F. S. Tedesco, M. Corada, M. Trani, R. D'Antuono, Q. Millet, F. Orsenigo, B. G. Galvez, G. Cossu, E. Dejana

    A molecular thermometer for nanoparticles for optical hyperthermia - Nano Lett (2013) vol. 13 pp2004-10
    S. Freddi, L. Sironi, R. D'Antuono, D. Morone, A. Dona, E. Cabrini, L. D'Alfonso, M. Collini, P. Pallavicini, G. Baldi, D. Maggioni, G. Chirico



    Acknowledgments:

    Exploitation of the Secretory Pathway by SARS-CoV-2 Envelope
    Pearson G. J. et al., https://www.biorxiv.org/content/10.1101/2021.06.30.450614v1

    Optimization of Metabolic Oligosaccharide Engineering with Ac4GalNAlk and Ac4GlcNAlk by an Engineered Pyrophosphorylase
    Cioce A. et al., ACS Chem. Biol. 2021, 16, 10, 1961–1967

    Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes
    Ivanova D. et al., Science advances 2021, vol. 7,18 eabf3873

    Macrophage-specific responses to human- and animal-adapted tubercle bacilli reveal pathogen and host factors driving multinucleated cell formation
    Queval C. J. et al., PLoS Pathog. 2021 Mar; 17(3): e1009410

    Pathogenic ACVR1R206H activation by Activin A‐induced receptor clustering and autophosphorylation
    Ramachandran A., Mehić M. et al., J Exp Med 6 July 2020; 217 (7): e20191933

    Reconstitution of a functional human thymus by postnatal stromal progenitor cells and natural whole-organ scaffolds
    Campinoti S. et al., Nat Commun 11, 6372 (2020)

    Modular microfluidics enables kinetic insight from time-resolved cryo-EM
    Mäeots M., Lee B., Nans A. et al., Nat Commun 11, 3465 (2020)

    Restriction of memory B cell differentiation at the germinal center B cell positive selection stage
    Toboso-Navasa A., Gunawan A., Morlino G. et al., J Exp Med 6 July 2020; 217 (7): e20191933

    A trainable clustering algorithm based on shortest paths from density peaks
    Pizzagalli et al., Science Advances 30 Oct 2019: Vol. 5, no. 10, eaax3770

    Influenza Vaccination Induces NK-Cell-Mediated Type-II IFN Response that Regulates Humoral Immunity in an IL-6-Dependent Manner
    Farsakoglu et al., Cell Reports, Vol.26, Issue 9 (2019)

    P2X7 receptor restrains pathogenic Tfh cell generation in systemic lupus erythematosus
    Faliti C.E. et al., J. Exp. Med. 2019 Vol. 216 No. 2 317–336

    A shortest-path based clustering algorithm for joint human-machine analysis of complex datasets
    Pizzagalli et al., https://arxiv.org/abs/1812.11850

    IL-23 secreted by myeloid cells drives castration-resistant prostate cancer
    Calcinotto et al., Nature, Vol.559 (2018)

    Redox-Mediated Mechanisms Fuel Monocyte Responses to CXCL12/HMGB1 in Active Rheumatoid Arthritis
    Cecchinato et al., Front. Immunol.(2018)

    Leukocyte Tracking Database, a collection of immune cell tracks from intravital 2-photon microscopy videos
    Pizzagalli D.U., Scientific Data volume 5, Article number: 180129 (2018)

    A Human Bi-specific Antibody against Zika Virus with High Therapeutic Potential
    Wang et al., Cell, Vol. 171, Issue 1, 21 September 2017, Pages 229-241.e15

    Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization
    Cecchinato et al., J Immunol January 1, 2017, 198 (1) 184-195